Flag-Tag mouse monoclonal antibody

Product Specifications

Background

Epitope tags are useful for the labeling and detection of proteins using immunoblotting, immunoprecipitation and immunostaining techniques. Due to their small size, they are unlikely to affect the tagged protein's biochemical properties.

Structure Composition

1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Product Name Alternative

DDDDK epitope tag; DYKDDDDK epitope tag; DYKDDDDK tag; ECS epitope tag, ECS tag; Enterokinase Cleavage Site epitope tag; Enterokinase Cleavage Site tag; DDDDK

Reactivity

Transfected

Immunogen

Synthetic peptide Flag-tag.

Conjugation

Unconjugated

Applications

WB IHC IF IP

Dilution

WB: 1:20000~1:100000 IHC/IF: 1:50~1:200 IP: 1:50~1:200

Purity

The antibody was affinity-purified from cell culture supernatant by protein A+G and the purity is > 95% (by SDS-PAGE) .

Modification

Unmodified

Storage Conditions

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Notes

For research use only, not for use in diagnostic procedure.

Specificity

Flag-Tag mAb detects over-expressed or recombinant proteins containing the Flag epitope tag.

Applications Notes

Western blot (WB) analysis of Flag-Tag mAb at 1:100000 dillution Lane1:HEK293F whole cell lysate , untransfected Lane2:HEK293F whole cell lysate , transfected (M-Flag) Lane3:HEK293F whole cell lysate , transfected (N-Flag) Lane4:HEK293F whole cell lysate , transfected (C-Flag)