Actin (muscle) Antibody
Mouse Monoclonal to Actin.
Product Specifications
Product Name Alternative
Muscle specific actin
Reactivity
Canine, Feline, Gallus, Human, Mouse, Primate, Rabbit, Rat
Immunogen
SDS extracted protein fraction of human myocardium
Target
Actin (muscle)
Clonality
Monoclonal
Clone
HHF35
Conjugation
Unconjugated
Field of Research
Musculoskeletal & Connective Tissue Research
Purification
Purified by protein-A affinity chromatography.
Concentration
1 mg/ml
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
Western blotting: Recommended dilution: 1 μg/ml; positive control: murine femoral muscle, murine heart, negative control: HUVEC line; reducing conditions. Immunohistochemistry (paraffin sections) : Antigen retrieval steps generally not required, but e.g. in case of arterial smooth muscle cells or myoepithelial cells, pepsin or trypsin pretreatment is recommended.
Tested Applications
ICC, IHC-Fr, IHC-P, WB
Preservative
Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
Isotype
Mouse IgG1
Entrez
35
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