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Actin (muscle) Antibody

Mouse Monoclonal to Actin.

Product Specifications

Product Name Alternative

Muscle specific actin

Reactivity

Canine, Feline, Gallus, Human, Mouse, Primate, Rabbit, Rat

Immunogen

SDS extracted protein fraction of human myocardium

Target

Actin (muscle)

Clonality

Monoclonal

Clone

HHF35

Conjugation

Unconjugated

Field of Research

Musculoskeletal & Connective Tissue Research

Purification

Purified by protein-A affinity chromatography.

Concentration

1 mg/ml

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Notes

For research use only.

Applications Notes

Western blotting: Recommended dilution: 1 μg/ml; positive control: murine femoral muscle, murine heart, negative control: HUVEC line; reducing conditions. Immunohistochemistry (paraffin sections) : Antigen retrieval steps generally not required, but e.g. in case of arterial smooth muscle cells or myoepithelial cells, pepsin or trypsin pretreatment is recommended.

Tested Applications

ICC, IHC-Fr, IHC-P, WB

Preservative

Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Isotype

Mouse IgG1

Entrez

35

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