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HLA-G Antibody

Mouse Monoclonal to HLA-G.

Product Specifications

UniProt

P17693

Reactivity

Human

Immunogen

Recombinant human HLA-G refolded with beta2-microglobulin and peptide.

Target

HLA-G

Clonality

Monoclonal

Clone

MEM-G/9

Conjugation

Unconjugated

Field of Research

Cell Biology

Purification

Purified by protein-A affinity chromatography.

Concentration

1 mg/ml

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Notes

For research use only.

Applications Notes

Flow cytometry: Recommended dilution: 0.3-4 μg/ml; positive control: JEG-3 human choriocarcinoma cell line. Immunocytochemistry: Recommended dilution: 2-5 μg/ml. ELISA: The antibody MEM-G/9 has been tested as the capture antibody in a sandwich ELISA for analysis of human HLA-G in combination with antibody B2M-01 or with antibody W6/32. Coating antibody 10 μg/ml, detection antibody (biotin or peroxidase conjugate) 1 μg/ml. Immunohistochemistry: Recommended dilution: 1-4 μg/ml.

Tested Applications

ELISA, FC, ICC, IHC-Fr, IP

Preservative

Phosphate buffered saline (PBS), pH 7.4

Isotype

Mouse IgG1

Entrez

3135

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