HLA-G Antibody
Mouse Monoclonal to HLA-G.
Product Specifications
UniProt
P17693
Reactivity
Human
Immunogen
Recombinant human HLA-G refolded with beta2-microglobulin and peptide.
Target
HLA-G
Clonality
Monoclonal
Clone
MEM-G/9
Conjugation
Unconjugated
Field of Research
Cell Biology
Purification
Purified by protein-A affinity chromatography.
Concentration
1 mg/ml
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
Flow cytometry: Recommended dilution: 0.3-4 μg/ml; positive control: JEG-3 human choriocarcinoma cell line. Immunocytochemistry: Recommended dilution: 2-5 μg/ml. ELISA: The antibody MEM-G/9 has been tested as the capture antibody in a sandwich ELISA for analysis of human HLA-G in combination with antibody B2M-01 or with antibody W6/32. Coating antibody 10 μg/ml, detection antibody (biotin or peroxidase conjugate) 1 μg/ml. Immunohistochemistry: Recommended dilution: 1-4 μg/ml.
Tested Applications
ELISA, FC, ICC, IHC-Fr, IP
Preservative
Phosphate buffered saline (PBS), pH 7.4
Isotype
Mouse IgG1
Entrez
3135
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