FYN Antibody
Mouse Monoclonal to Fyn.
Product Specifications
Product Name Alternative
P59Fyn, SLK, SYN
UniProt
P06241
Reactivity
Human, Mouse
Immunogen
Bacterially expressed recombinant fragment of human Fyn (aa 7-176) .
Target
FYN
Clonality
Monoclonal
Clone
FYN-01
Conjugation
Unconjugated
Field of Research
Signal Transduction
Purification
Purified by protein-A affinity chromatography.
Concentration
1 mg/ml
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
Immunohistochemistry (paraffin sections) : Recommended dilution: 5 μg/ml; positive tissue: brain. Western blotting: Recommended dilution: 2 μg/ml; positive control: JURKAT human T cell leukemia cell line. Immunoprecipitation: positive control: J77 T cell line. Preparation of cell lysate: 30 min on ice (orbital incubator) in lysing buffer, lysing buffer with N-dodecyl beta-D-maltoside (20 mM Tris/Cl, 100 mM NaCl pH 8,2, 1% laurylmaltosid (w/v), 50 mM NaF) . The antibody FYN-01 apparently gives very specific signal (by 59 kDa), and nicely immunoprecipitates Fyn from cell lysates.
Tested Applications
ICC, IHC-P, IP, WB
Preservative
Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
Isotype
Mouse IgG2b
Entrez
2534
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