Mouse IgM Isotype Control Purified Antibody
Mouse IgM Isotype Control
Product Specifications
Clonality
Monoclonal
Clone
PFR-03
Conjugation
Unconjugated
Purification
Purified by sequential steps of physicochemical fractionation (differential precipitation and solid-phase chromatography methods) .
Concentration
1 mg/ml
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
Negative control: The reagent is intended as an isotype control to establish the amount of non-specific antibody binding. For your particular experiment, use the same concentration of this control antibody as the recommended working concentration of the antigen-specific antibody. Also, when working with prediluted antibodies, dilute the isotype control to the same concentration as is the concentration of the antigen-specific antibody in the prediluted antibody solution you are using. If under particular experimental conditions the background signal of the isotype control is too high (usually when working concentrations of used antibodies are above 10 μg/ml of incubation mixture), change the conditions of your experiment to reduce the background.
Tested Applications
ELISA, FC, ICC, IHC-Fr, IHC-P, IP, WB
Preservative
Tris buffered saline (TBS), pH 8.0, 15 mM sodium azide
Isotype
Mouse IgM
Entrez
35
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