SingleFlowEx CD45 Pacific Blue™

SingleFlowEx is fluorochrome-labeled monoclonal antibody single reagent for use in laboratory-developed multicolor flow cytometry tests in order to examine human clinical samples. SingleFlowEx can be used in various multicolor combinations. Laboratory shall establish the SingleFlowEx performance characteristics in conjunction with other reagents in normal and pathological samples.

Product Specifications

Certification

RUO (GMP)

Clone

2D1

Applications

FC (QC tested)

Concentration

0.10 mg/mL

Format

Pacific Blue

Buffer

Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Components

1 vial of monoclonal antibody reagent.The content (1 mL) is sufficient for min. 100 Tests.

References & Citations

*Wellhausen SR, Slone SP, Miller JJ: Clone-specific anti-CD45 blocking factor in patient plasma. Cytometry B Clin Cytom. 2007 Sep;72 (5) :423-6., URL: https://www.ncbi.nlm.nih.gov/pubmed/17311353, *Janossy G, Jani IV, Bradley NJ, Bikoue A, Pitfield T, Glencross DK: Affordable CD4 (+) -T-cell counting by flow cytometry: CD45 gating for volumetric analysis. Clin Diagn Lab Immunol. 2002 Sep;9 (5) :1085-94., URL: https://www.ncbi.nlm.nih.gov/pubmed/12204964, *Ito A, Ishida T, Yano H, Inagaki A, Suzuki S, Sato F, Takino H, Mori F, Ri M, Kusumoto S, Komatsu H, Iida S, Inagaki H, Ueda R: Defucosylated anti-CCR4 monoclonal antibody exercises potent ADCC-mediated antitumor effect in the novel tumor-bearing humanized NOD/Shi-scid, IL-2Rgamma (null) mouse model. Cancer Immunol Immunother. 2009 Aug;58 (8) :1195-206., URL: https://pubmed.ncbi.nlm.nih.gov/19048251/, *Pfau JC, Walker E, Card GL: Monoclonal antibodies to CD45 modify LPS-induced arachidonic acid metabolism in macrophages. Biochim Biophys Acta. 2000 Feb 28;1495 (3) :212-22., URL: https://www.ncbi.nlm.nih.gov/pubmed/10699460, *Mariucci S, Rovati B, Bencardino K, Manzoni M, Danova M: Flow cytometric detection of circulating endothelial cells and endothelial progenitor cells in healthy subjects. Int J Lab Hematol. 2010 Feb;32 (1 Pt 1) :e40-8., URL: https://www.ncbi.nlm.nih.gov/pubmed/20088999, *Vicetti Miguel RD, Harvey SA, LaFramboise WA, Reighard SD, Matthews DB, Cherpes TL: Human female genital tract infection by the obligate intracellular bacterium Chlamydia trachomatis elicits robust Type 2 immunity. PLoS One. 2013;8 (3) :e58565., URL: https://www.ncbi.nlm.nih.gov/pubmed/23555586, *Sutherland DR, Illingworth A, Marinov I, Ortiz F, Andreasen J, Payne D, Wallace PK, Keeney M: ICCS/ESCCA consensus guidelines to detect GPI-deficient cells in paroxysmal nocturnal hemoglobinuria (PNH) and related disorders part 2 - reagent selection and assay optimization for high-sensitivity testing. Cytometry B Clin Cytom. 2018 Jan;94 (1) :23-48., URL: https://www.ncbi.nlm.nih.gov/pubmed/29236353, *Use of this product has not been published., URL:

Other References

*Li FJ, Tsuyama N, Ishikawa H, Obata M, Abroun S, Liu S, Otsuyama K, Zheng X, Ma Z, Maki Y, Kawano MM: A rapid translocation of CD45RO but not CD45RA to lipid rafts in IL-6-induced proliferation in myeloma. Blood. 2005 Apr 15;105 (8) :3295-302. , URL: https://www.ncbi.nlm.nih.gov/pubmed/15626731?ordin, *Cosenza-Nashat MA, Kim MO, Zhao ML, Suh HS, Lee SC: CD45 isoform expression in microglia and inflammatory cells in HIV-1 encephalitis. Brain Pathol. 2006 Oct;16 (4) :256-65., URL: http://www.ncbi.nlm.nih.gov/pubmed/17107594?ordina, *Dawes R, Petrova S, Liu Z, Wraith D, Beverley PC, Tchilian EZ. Combinations of CD45 isoforms are crucial for immune function and disease. J Immunol. 2006 Mar 15;176 (6) :3417-25., URL: http://www.ncbi.nlm.nih.gov/pubmed/16517710?ordina, *Bijian K, Zhang L, Shen SH: Collagen-mediated survival signaling is modulated by CD45 in Jurkat T cells. Mol Immunol. 2007 Jul;44 (15) :3682-90., URL: http://www.ncbi.nlm.nih.gov/pubmed/17524482?ordina, *Desharnais P, Dupéré-Minier G, Hamelin C, Devine P, Bernier J: Involvement of CD45 in DNA fragmentation in apoptosis induced by mitochondrial perturbing agents. Apoptosis. 2007 Dec 19. , URL: http://www.ncbi.nlm.nih.gov/pubmed/18157742?ordina