Mouse DPYSL3 (Dihydropyrimidinase Like Protein 3) ELISA Kit

Collapsin response mediator protein 4; CRMP 4 ; CRMP-4; CRMP4; Dihydropyrimidinase like 3 ; Dihydropyrimidinase related protein 3; Dihydropyrimidinase-related protein 3; DPYL3; DPYSL3; DRP 3 ; DRP-3; DRP3; LCRMP; TUC4; ULIP 1; ULIP; ULIP-1; ULIP1; UNc 33 like phosphoprotein 1; Unc 33 like phosphoprotein; Unc-33-like phosphoprotein 1

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse DPYSL3. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse DPYSL3. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse DPYSL3, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse DPYSL3 in the samples is then determined by comparing the OD of the samples to the standard curve.