Pig PAI1 (Plasminogen Activator Inhibitor 1) ELISA Kit

SERPINE1; PLANH1; Serpin Peptidase Inhibitor Clade E Member 1; Nexin, Plasminogen Activator Inhibitor Type 1 Serpin E1; Endothelial plasminogen activator inhibitor

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Pig PAI1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Pig PAI1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Pig PAI1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Pig PAI1 in the samples is then determined by comparing the OD of the samples to the standard curve.