VTG | Sole vitellogenin Antibody

Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles, Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes

The developed VTG ELISA, using these VTG and AbVTG, has been validated for Senegalese sole, sea bass (Dicentrarchus labrax) and seabream (Sparus aurata), which all gave parallel displacement curves in the assay. Probably, plasmas from several other fish species displace parallel and can also be used in the assay, although it has to be validated for each case. VTG can be purified using following methodology: Mañanós et al. (1994) . Sea bass (Dicentrarchus labrax L.) vitellogenin. I—Induction, purification and partial characterization. Comparative Biochemistry and Physiology Part B: Comparative Biochemistry, Vol 107 (2) : 205-216.Guzman et al. (2008) Vitellogenin, steroid plasma levels and spawning performance of cultured female Senegalese sole (Solea senegalensis) .Gen and Comp Endocrinology 156: 285-297