Recombinant Human IL-5
Product Specifications
Product Name Alternative
Interleukin-5, IL-5, B-cell differentiation factor I, Eosinophil differentiation factor, T-cell replacing factor, TRF, IL5
UniProt
P05113
Expression Region
20-134
Host
Mammalian Cells
Tag
C-terminal His Tag
Applications
Western Blot, ELISA
Endotoxin
<1 EU/µg as determined by LAL test.
Purity
Greater than 95% as determined by SDS-PAGE.
Activity
Measured in a cell proliferation assay using TF_x001E_1 human erythroleukemic cells. The ED50 for this effect is 0.1-0.5 ng/ml.
Form
Lyophilized powder
Reconstitution
Centrifuge the vial at 10000 rpm for 30 s before opening, reconstitute in sterile distilled water to a concentration of 0.1-1 mg/ml by gently pipetting 2-3 times, don't vortex.
Molecular Weight
20 kDa
Shipping Conditions
4°C with ice bag
Storage Conditions
The lyophilized protein is stable at -20 °C for up to 1 year. After reconstitution, the protein solution is stable at -20 to -80 °C for 3 months or 2-7 days at 2 to 8 °C under sterile conditions. For extended storage, it is recommended to further dilute in working aliquots, avoid repeated freeze/thaw cycle.
Formulation
Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.
Organism Species
Human
Available Sizes
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