Recombinant Human IL-5

Product Specifications

Product Name Alternative

Interleukin-5, IL-5, B-cell differentiation factor I, Eosinophil differentiation factor, T-cell replacing factor, TRF, IL5

UniProt

P05113

Expression Region

20-134

Host

Mammalian Cells

Tag

C-terminal His Tag

Applications

Western Blot, ELISA

Endotoxin

<1 EU/µg as determined by LAL test.

Purity

Greater than 95% as determined by SDS-PAGE.

Activity

Measured in a cell proliferation assay using TF_x001E_1 human erythroleukemic cells. The ED50 for this effect is 0.1-0.5 ng/ml.

Form

Lyophilized powder

Reconstitution

Centrifuge the vial at 10000 rpm for 30 s before opening, reconstitute in sterile distilled water to a concentration of 0.1-1 mg/ml by gently pipetting 2-3 times, don't vortex.

Molecular Weight

20 kDa

Shipping Conditions

4°C with ice bag

Storage Conditions

The lyophilized protein is stable at -20 °C for up to 1 year. After reconstitution, the protein solution is stable at -20 to -80 °C for 3 months or 2-7 days at 2 to 8 °C under sterile conditions. For extended storage, it is recommended to further dilute in working aliquots, avoid repeated freeze/thaw cycle.