Recombinant Mouse EGF
Product Specifications
Product Name Alternative
Beta urogastrone, beta-urogastrone, EGF, EGF_HUMAN, Epidermal growth factor, HOMG4, OTTHUMP00000219721, OTTHUMP00000219722, Pro epidermal growth factor, URG, Urogastrone
UniProt
P01132
Expression Region
977-1029
Host
E.Coli
Tag
C-terminal His Tag
Applications
Western Blot, ELISA
Endotoxin
< 1 EU/µg as determined by LAL test.
Purity
Greater than 90% as determined by SDS-PAGE.
Activity
Measured by its ability to inhibits the proliferation of human epithelial A431 cells.The ED50 for this effect is 2-20 ng/ml (QC verified) .
Form
Lyophilized powder
Reconstitution
Centrifuge the vial prior to opening, reconstitute in sterile distilled water or buffer of interest to concentration of 0.1-1 mg/ml by gently pipetting 2-3 times, don't vortex.
Molecular Weight
9-14 kDa
Shipping Conditions
4°C with ice bag
Storage Conditions
The lyophilized protein is stable at -20 °C for up to 1 year. For extended storage, it is recommended to further dilute in working aliquots after reconstitution. The protein solution is stable at ≤ -20°C for 3 months, or 2-7 days at 2-8 °C under sterile conditions.Avoid repeated freeze/thaw cycle.
Formulation
Lyophilized from a 0.2 μm filtered solution of 20mM Tris-HCl, 150mM NaCl, pH 8.0.
Organism Species
Mouse
Available Sizes
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