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Recombinant Mouse EGF

Product Specifications

Product Name Alternative

Beta urogastrone, beta-urogastrone, EGF, EGF_HUMAN, Epidermal growth factor, HOMG4, OTTHUMP00000219721, OTTHUMP00000219722, Pro epidermal growth factor, URG, Urogastrone

UniProt

P01132

Expression Region

977-1029

Host

E.Coli

Tag

C-terminal His Tag

Applications

Western Blot, ELISA

Endotoxin

< 1 EU/µg as determined by LAL test.

Purity

Greater than 90% as determined by SDS-PAGE.

Activity

Measured by its ability to inhibits the proliferation of human epithelial A431 cells.The ED50 for this effect is 2-20 ng/ml (QC verified) .

Form

Lyophilized powder

Reconstitution

Centrifuge the vial prior to opening, reconstitute in sterile distilled water or buffer of interest to concentration of 0.1-1 mg/ml by gently pipetting 2-3 times, don't vortex.

Molecular Weight

9-14 kDa

Shipping Conditions

4°C with ice bag

Storage Conditions

The lyophilized protein is stable at -20 °C for up to 1 year. For extended storage, it is recommended to further dilute in working aliquots after reconstitution. The protein solution is stable at ≤ -20°C for 3 months, or 2-7 days at 2-8 °C under sterile conditions.Avoid repeated freeze/thaw cycle.

Formulation

Lyophilized from a 0.2 μm filtered solution of 20mM Tris-HCl, 150mM NaCl, pH 8.0.

Organism Species

Mouse

Available Sizes

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