PE Anti-Mouse CD4 Antibody (RM4-5)
Product Specifications
Background
CD4 is a 55 kD protein also known as L3T4 or T4. It is a member of the Ig superfamily, primarily expressed on most thymocytes and a subset of T cells, and weakly on macrophages and dendritic cells. It acts as a co-receptor with the TCR during T cell activation and thymic differentiation by binding MHC class II and associating with the protein tyrosine kinase lck.
Synonyms
L3T4| T-cell surface antigen T4/Leu-3| T-cell surface glycoprotein CD4| T4
Gene ID
12504
UniProt
P06332
Host
Rat
Reactivity
Mouse
Target
Mouse CD4
Clonality
Monoclonal
Isotype
IgG2a κ
Conjugation
PE
Dilution
Each lot of this antibody is quality control tested by flow cytometric analysis. Please check your vial before the experiment. Since applications vary, the appropriate dilutions must be determined for individual use. We suggest each investigator should titrate the reagent to obtain optimal results [The recommended concentration is 0.1-1μg/10⁶ cells in 100 μL volume].
Form
Liquid
Shipping Conditions
4°C with ice bag
Storage Conditions
PBS with 0. 1% sodium azide, 1%BSA, pH 7.3, 2-8°C for 12 months (Avoid repeated freeze / thaw cycles.)
Clone ID
RM4-5
Tested Applications
FC
Conjugation Information
PE is designed to be excited by the Blue (488 nm), Green (532 nm) and Yellow-Green (561 nm) lasers and detected using an optical filter centered near 575 nm (e.g., a 585/42 nm bandpass filter) .
Available Sizes
Curated Selection
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