FITC Anti-Mouse CD4 Antibody (RM4-5)

Product Specifications

Background

CD4 is a 55 kD protein also known as L3T4 or T4. It is a member of the Ig superfamily, primarily expressed on most thymocytes and a subset of T cells, and weakly on macrophages and dendritic cells. It acts as a co-receptor with the TCR during T cell activation and thymic differentiation by binding MHC class II and associating with the protein tyrosine kinase lck.

Synonyms

L3T4| T-cell surface antigen T4/Leu-3| T-cell surface glycoprotein CD4| T4

Gene ID

12504

UniProt

P06332

Host

Rat

Reactivity

Mouse

Target

Mouse CD4

Clonality

Monoclonal

Isotype

IgG2a κ

Conjugation

FITC

Dilution

Each lot of this antibody is quality control tested by flow cytometric analysis. Please check your vial before the experiment. Since applications vary, the appropriate dilutions must be determined for individual use. We suggest each investigator should titrate the reagent to obtain optimal results [The recommended concentration is 0.1-1μg/10⁶ cells in 100 μL volume].

Form

Liquid

Shipping Conditions

4°C with ice bag

Storage Conditions

PBS with 0. 1% sodium azide, 1%BSA, pH 7.3, 2-8°C for 12 months (Avoid repeated freeze / thaw cycles.)

Clone ID

RM4-5

Tested Applications

Conjugation Information

FITC is designed to be excited by the Blue laser (488 nm) and detected using an optical filter centered near 530 nm (e.g., a 525/40 nm bandpass filter) .