FineTest®647 Anti-Mouse H-2 Antibody (M1/42)

Product Specifications

Background

The M1/42 antibody reacts with the H-2 MHC class I alloantigens expressed on nucleated cells from mice of the a, b, d, j, k, s, and u haplotypes (Stallcup, KC et al, 1981) . MHC class I is involved in antigen presentation to T cells expressing CD3/TCR and CD8 proteins.

Synonyms

MHC I| Mouse major histocompatibility complex (MHC) H-2

Gene ID

111364

UniProt

P06345

Host

Rat

Reactivity

Mouse

Target

Mouse H-2

Clonality

Monoclonal

Isotype

IgG2a κ

Conjugation

FineTest®647

Dilution

Each lot of this antibody is quality control tested by flow cytometric analysis. Please check your vial before the experiment. Since applications vary, the appropriate dilutions must be determined for individual use. We suggest each investigator should titrate the reagent to obtain optimal results [The recommended concentration is 0.1-1μg/10⁶ cells in 100 μL volume].

Form

Liquid

Shipping Conditions

4°C with ice bag

Storage Conditions

PBS with 0. 1% sodium azide, 1%BSA, pH 7.3, 2-8°C for 12 months (Avoid repeated freeze / thaw cycles.)

Clone ID

M1/42

Tested Applications

Conjugation Information

FineTest® 647 is designed to be excited by the Red laser (627-640 nm) and detected using an optical filter centered near 670 nm (e.g., a 660/20 nm bandpass filter) .