PerCP-Cy5.5 Anti-Mouse CD14 Antibody (Sa14-2)

Product Specifications

Background

CD14 is a 53-55 kD glycosylphosphatidylinositol (GPI) -linked membrane glycoprotein also known as LPS receptor. CD14 is expressed on macrophages, dendritic cells, Kupffer cells, hepatocytes, and granulocytes. As a high-affinity receptor for LPS-LBP (LPS-binding protein) complex, CD14, in association with Toll-like Receptor 4 (TLR4) or 2 (TLR2), is involved in the clearance of gram-negative pathogens.

Synonyms

CD 14| Monocyte differentiation antigen CD14| Myeloid cell-specific leucine-rich glycoprotein

Gene ID

12475

UniProt

P10810

Host

Rat

Reactivity

Mouse

Target

Mouse CD14

Clonality

Monoclonal

Isotype

IgG2a κ

Conjugation

PerCP-Cy5.5

Dilution

Each lot of this antibody is quality control tested by flow cytometric analysis. Please check your vial before the experiment. Since applications vary, the appropriate dilutions must be determined for individual use. We suggest each investigator should titrate the reagent to obtain optimal results [The recommended concentration is 0.1-1μg/10⁶ cells in 100 μL volume].

Form

Liquid

Shipping Conditions

4°C with ice bag

Storage Conditions

PBS with 0. 1% sodium azide, 1%BSA, pH 7.3, 2-8°C for 12 months (Avoid repeated freeze / thaw cycles.)

Clone ID

Sa14-2

Tested Applications

Conjugation Information

PerCP-Cy5.5 is designed to be excited by the blue laser (488 nm) and detected using an optical filter centered near 675 nm (e.g., a 690/50 nm bandpass filter) .