APC Anti-Mouse CD3ε Antibody (145-2C11)

Product Specifications

Background

CD3ε is a 20 kD transmembrane protein, also known as CD3 or T3. It is a member of the Ig superfamily and primarily expressed on T cells, NK-T cells, and at different levels on thymocytes during T cell differentiation. CD3ε forms a TCR complex by associating with the CD3δ, γ and ζ chains, as well as the TCR α/β or γ/δ chains. CD3 plays a critical role in TCR signal transduction, T cell activation, and antigen recognition by binding the peptide/MHC antigen complex.

Synonyms

CD3E| CD3e| T-cell surface antigen T3/Leu-4 epsilon chain| T-cell surface glycoprotein CD3 epsilon chain| T3E

Gene ID

12501

UniProt

P22646

Host

Armenian Hamster

Reactivity

Mouse

Target

Mouse CD3ε

Clonality

Monoclonal

Isotype

IgG

Conjugation

APC

Dilution

Each lot of this antibody is quality control tested by flow cytometric analysis. Please check your vial before the experiment. Since applications vary, the appropriate dilutions must be determined for individual use. We suggest each investigator should titrate the reagent to obtain optimal results [The recommended concentration is 0.1-1μg/10⁶ cells in 100 μL volume].

Form

Liquid

Shipping Conditions

4°C with ice bag

Storage Conditions

PBS with 0. 1% sodium azide, 1%BSA, pH 7.3, 2-8°C for 12 months (Avoid repeated freeze / thaw cycles.)

Clone ID

145-2C11

Tested Applications

Conjugation Information

APC is designed to be excited by the Red (627-640 nm) laser and detected using an optical filter centered near 660 nm (e.g., a 660/20 nm bandpass filter) .