APC Anti-Mouse CD3 Antibody (17A2)

Product Specifications

Background

CD3, also known as T3, is a member of the Ig superfamily and primarily expressed on T cells, NK-T cells, and at different levels on thymocytes during T cell differentiation. CD3 is composed of CD3ε, δ, γ and ζ chains. It forms a TCR complex by associating with TCR α/β or γ/δ chains. CD3 plays a critical role in TCR signal transduction, T cell activation, and antigen recognition by binding the peptide/MHC antigen complex.

Synonyms

CD3| CD3E/D/G/Z| CD3e/d/g/z| T-cell surface glycoprotein CD 3epsilon/delta/gamma/zeta chain

Gene ID

12502

UniProt

P04235, P11942, P22646, P24161

Host

Rat

Reactivity

Mouse

Target

Mouse CD3

Clonality

Monoclonal

Isotype

IgG2b κ

Conjugation

APC

Dilution

Each lot of this antibody is quality control tested by flow cytometric analysis. Please check your vial before the experiment. Since applications vary, the appropriate dilutions must be determined for individual use. We suggest each investigator should titrate the reagent to obtain optimal results [The recommended concentration is 0.1-1μg/10⁶ cells in 100 μL volume].

Form

Liquid

Shipping Conditions

4°C with ice bag

Storage Conditions

PBS with 0. 1% sodium azide, 1%BSA, pH 7.3, 2-8°C for 12 months (Avoid repeated freeze / thaw cycles.)

Clone ID

17A2

Tested Applications

Conjugation Information

APC is designed to be excited by the Red (627-640 nm) laser and detected using an optical filter centered near 660 nm (e.g., a 660/20 nm bandpass filter) .