PE-Cy5 Anti-Mouse CD16/32 Antibody (2.4G2)

CD16 is low affinity IgG Fc receptor III (FcR III) and CD32 is FcR II. CD16/CD32 are expressed on B cells, monocytes/macrophages, NK cells, granulocytes, mast cells, and dendritic cells. The Fc receptors bind antibody-antigen immune complexes and mediate adaptive immune responses.

CD16a/b| CD32| CD32A/B| FCG2A| FCGR2A/BFCGR3| FCGR3A/B| Fc fragment of IgG low affinity IIIa/b receptor| Fc fragment of IgG low affinity IIIb receptor| Fc fragment of IgG low affinity IIa/b receptor| Fc gamma RIIa/bFc gamma receptor III A/B| FcGR

Each lot of this antibody is quality control tested by flow cytometric analysis. Please check your vial before the experiment. Since applications vary, the appropriate dilutions must be determined for individual use. We suggest each investigator should titrate the reagent to obtain optimal results [The recommended concentration is 0.1-1μg/10⁶ cells in 100 μL volume].

PE-Cy5 is designed to be excited by the Blue (488 nm), Green (532 nm) and yellow-green (561 nm) lasers and detected using an optical filter centered near 670 nm (e.g., a 690/50 nm bandpass filter) .