PE-Cy5.5 Anti-Mouse MHC II (I-A/I-E) Antibody (M5/114)

Product Specifications

Background

These class II molecules are expressed on antigen presenting cells (including B cells) and a subset of T cells from H-2b, d, q, r bearing mice and are involved in antigen presentation to T cells expressing CD3/TCR and CD4 proteins.

Synonyms

H2-Ab1/Eb1| I-E beta MHC class II| MHC class II| MHC class II H2-IA-beta-psi| Major histocompatibility protein class II beta chain

Gene ID

14961, 14969

UniProt

P14483, O78196

Host

Rat

Reactivity

Mouse

Target

Mouse MHC II (I-A/I-E)

Clonality

Monoclonal

Isotype

IgG2b κ

Conjugation

PE-Cy5.5

Dilution

Each lot of this antibody is quality control tested by flow cytometric analysis. Please check your vial before the experiment. Since applications vary, the appropriate dilutions must be determined for individual use. We suggest each investigator should titrate the reagent to obtain optimal results [The recommended concentration is 0.1-1μg/10⁶ cells in 100 μL volume].

Form

Liquid

Shipping Conditions

4°C with ice bag

Storage Conditions

PBS with 0. 1% sodium azide, 1%BSA, pH 7.3, 2-8°C for 12 months (Avoid repeated freeze / thaw cycles.)

Clone ID

M5/114

Tested Applications

Conjugation Information

PE-Cy5.5 is designed to be excited by the Blue (488 nm), Green (532 nm) and yellow-green (561 nm) lasers and detected using an optical filter centered near 690 nm (e.g., a 690/50 nm bandpass filter) .