Pacific Blue Anti-mouse Lineage Cocktail

The mouse lineage panel has been designed to react with cells from the major hematopoietic cell lineages, such as T lymphocytes, B lymphocytes, monocytes/macrophages, granulocytes, NK cells, and erythrocytes. The Pacific Blue Mouse Lineage Antibody Cocktail is designed for the flow cytometric identification of hematopoietic progenitors in mouse bone marrow. Components include anti-mouse CD3, clone 17A2; anti-mouse Ly-6G/Ly-6C, clone RB6-8C5; anti-mouse CD11b, clone M1/70; anti-mouse CD45R/B220, clone RA3.3A1/6.1; anti-mouse TER-119, clone Ter-119.

Each lot of these antibodies is quality control tested by flow cytometric analysis. Volume per test: 5μL. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µL volume or 5 µl per 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

Pacific Blue is designed to be excited by the violet (405 nm) lasers and detected using an optical filter centered near 455 nm (e.g., a 450/50 nm bandpass filter) .