FineTest®700 Anti-Mouse IL-10 Antibody (JES5-16E3)

IL-10 was originally described as Cytokine Synthesis Inhibitory Factor (CSIF) by virtue of its ability to inhibit cytokine production by Th1 clones. IIL-10 shares over 80% sequence homology with the Epstein-Barr virus protein BCRFI. IIL-10 inhibits IFN-γ, TNF-β, and IL-2 production by Th1 clones; inhibits macrophage-mediated IL-1, IL-6, and TNF-α synthesis; suppresses the delayed type hypersensitivity response; stimulates Th2 cell response (which results in elevated antibody production) ; and promotes mast cell proliferation in combination with IL-4.

Volume per test: 5μL. Each lot of this antibody is quality control tested by flow cytometric analysis. The amount of the reagent is suggested to be used 5 µL of antibody per test (million cells in 100 µL staining volume or per 100 µL of whole blood) . Please check your vial before the experiment. Since applications vary, the appropriate dilutions must be determined for individual use.

FineTest® 700 is designed to be excited by the Red laser (627-640 nm) and detected using an optical filter centered near 719 nm (e.g., a 725/40 nm bandpass filter) .