FineTest®Violet 540 Anti-Mouse MHC II (I-A/I-E) Antibody (M5/114)

Product Specifications

Background

These class II molecules are expressed on antigen presenting cells (including B cells) and a subset of T cells from H-2b, d, q, r bearing mice and are involved in antigen presentation to T cells expressing CD3/TCR and CD4 proteins.

Synonyms

H2-Ab1/Eb1|I-E beta MHC class II|MHC class II|MHC class II H2-IA-beta-psi|Major histocompatibility protein class II beta chain

Gene ID

14961, 14969

UniProt

P14483, O78196

Host

Rat

Reactivity

Mouse

Target

Mouse MHC II

Clonality

Monoclonal

Isotype

IgG2b κ

Conjugation

FineTest®Violet 540

Dilution

Volume per test: 5μL. Each lot of this antibody is quality control tested by flow cytometric analysis. The amount of the reagent is suggested to be used 5 µL of antibody per test (million cells in 100 µL staining volume or per 100 µL of whole blood) . Please check your vial before the experiment. Since applications vary, the appropriate dilutions must be determined for individual use.

Form

Liquid

Shipping Conditions

4°C with ice bag

Storage Conditions

PBS with 0. 1% sodium azide, 1%BSA, pH 7.3, 2-8°C for 12 months (Avoid repeated freeze / thaw cycles.)

Clone ID

M5/114

Tested Applications

Conjugation Information

FineTest®Violet 540 is designed to be excited by the violet laser (405 nm) and detected using an optical filter centered near 548 nm (e.g., a 572/28 nm bandpass filter) .