FineTest®Violet 540 Anti-Mouse CD3 Antibody (17A2)

Product Specifications

Background

CD3, also known as T3, is a member of the Ig superfamily and primarily expressed on T cells, NK-T cells, and at different levels on thymocytes during T cell differentiation. CD3 is composed of CD3ε, δ, γ and ζ chains. It forms a TCR complex by associating with TCR α/β or γ/δ chains. CD3 plays a critical role in TCR signal transduction, T cell activation, and antigen recognition by binding the peptide/MHC antigen complex.

Synonyms

CD3|CD3E/D/G/Z|CD3e/d/g/z|T-cell surface glycoprotein CD 3epsilon/delta/gamma/zeta chain

Gene ID

12502

UniProt

P04235, P11942, P22646, P24161

Host

Rat

Reactivity

Mouse

Target

Mouse CD3

Clonality

Monoclonal

Isotype

IgG2b κ

Conjugation

FineTest®Violet 540

Dilution

Volume per test: 5μL. Each lot of this antibody is quality control tested by flow cytometric analysis. The amount of the reagent is suggested to be used 5 µL of antibody per test (million cells in 100 µL staining volume or per 100 µL of whole blood) . Please check your vial before the experiment. Since applications vary, the appropriate dilutions must be determined for individual use.

Form

Liquid

Shipping Conditions

4°C with ice bag

Storage Conditions

PBS with 0. 1% sodium azide, 1%BSA, pH 7.3, 2-8°C for 12 months (Avoid repeated freeze / thaw cycles.)

Clone ID

17A2

Tested Applications

Conjugation Information

FineTest®Violet 540 is designed to be excited by the violet laser (405 nm) and detected using an optical filter centered near 548 nm (e.g., a 572/28 nm bandpass filter) .