ATG7 antibody
Product Specifications
Background
E1-like activating enzyme involved in the 2 ubiquitin-like systems required for cytoplasm to vacuole transport (Cvt) and autophagy. Activates ATG12 for its conjugation with ATG5 as well as the ATG8 family proteins for their conjugation with phosphatidylethanolamine. Both systems are needed for the ATG8 association to Cvt vesicles and autophagosomes membranes. Required for autophagic death induced by caspase-8 inhibition. Required for mitophagy which contributes to regulate mitochondrial quantity and quality by eliminating the mitochondria to a basal level to fulfill cellular energy requirements and preventing excess ROS production. Modulates p53/TP53 activity to regulate cell cycle and survival during metabolic stress. Plays also a key role in the maintenance of axonal homeostasis, the prevention of axonal degeneration, the maintenance of hematopoietic stem cells, the formation of Paneth cell granules, as well as in adipose differentiation.
Synonyms
Ubiquitin-like modifier-activating enzyme ATG7|ATG12-activating enzyme E1 ATG7|Autophagy-related protein 7 (APG7-like, hAGP7) |Ubiquitin-activating enzyme E1-like protein|ATG7|APG7L
Gene ID
10533
UniProt
O95352
Host
Mouse
Reactivity
Human, Mouse, Rat
Immunogen
ATG7 autophagy related 7 homolog
Target
ATG7
Clonality
Monoclonal
Isotype
IgG2a
Field of Research
Immunology, Cardiovascular, Metabolism
Purification
Protein A+G purification
Dilution
WB: 1:1000-1:5000; IHC: 1:50-1:200; IF: 1:20-1:100
Purity
≥95% as determined by SDS-PAGE
Form
Liquid
Molecular Weight
68-78 kDa
Shipping Conditions
4°C with ice bag
Storage Conditions
PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20°C for 12 months (Avoid repeated freeze / thaw cycles.)
Clone ID
8H1
Tested Applications
ELISA, WB, IHC, IF
Curated Selection
Explore Other Products
Discover premium biology products from our extensive collection of 20M+ items
