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MYC-tag antibody

Product Specifications

Background

Protein tags are protein or peptide sequences located either on the C-or N-terminal of the target protein, which facilitates one or several of the following characteristics: solubility, detection, purification, localization and expression. The Myc tag corresponds to amino acid residues (EQKLISEEDL) of the human c-Myc protein. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits. Myc-Tag mouse mAb detects recombinant proteins containing the Myc tag. The antibody recognizes the Myc-tag EQKLISEEDL fused to either the amino-or carboxy-terminus of targeted proteins.

Synonyms

Myc|MYC-tag|EQKLISEEDL

Host

Mouse

Reactivity

Recombinant protein

Immunogen

MYC-tag

Target

MYC-tag

Clonality

Monoclonal

Isotype

IgG1

Purification

Protein A+G purification

Dilution

WB: 1:1000-1:5000; IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

Purity

≥95% as determined by SDS-PAGE

Form

Liquid

Shipping Conditions

4°C with ice bag

Storage Conditions

PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20°C for 12 months (Avoid repeated freeze / thaw cycles.)

Clone ID

6C4

Tested Applications

ELISA, WB, IP

Immunogen Sequence

Peptide

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