MYC-tag antibody
Product Specifications
Background
Protein tags are protein or peptide sequences located either on the C-or N-terminal of the target protein, which facilitates one or several of the following characteristics: solubility, detection, purification, localization and expression. The Myc tag corresponds to amino acid residues (EQKLISEEDL) of the human c-Myc protein. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits. Myc-Tag mouse mAb detects recombinant proteins containing the Myc tag. The antibody recognizes the Myc-tag EQKLISEEDL fused to either the amino-or carboxy-terminus of targeted proteins.
Synonyms
Myc|MYC-tag|EQKLISEEDL
Host
Mouse
Reactivity
Recombinant protein
Immunogen
MYC-tag
Target
MYC-tag
Clonality
Monoclonal
Isotype
IgG1
Purification
Protein A+G purification
Dilution
WB: 1:1000-1:5000; IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Purity
≥95% as determined by SDS-PAGE
Form
Liquid
Shipping Conditions
4°C with ice bag
Storage Conditions
PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20°C for 12 months (Avoid repeated freeze / thaw cycles.)
Clone ID
6C4
Tested Applications
ELISA, WB, IP
Immunogen Sequence
Peptide
Curated Selection
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