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HRP-GFP tag Monoclonal antibody

Product Specifications

Background

Green fluorescence protein (GFP) is a protein composed of 238 amino acid residues (26.9kDa) derived from the Jellyfish Aequorea victoria, which emits green light (emission peak at 509nm) when excited by blue light (excitation peak at 395nm) . GFP has become an invaluable tool in cell biology research, since its intrinsic fluorescence can be visualized in living cells.In contrast to wtGFP, EGFP has a single, strong, red-shifted excitation peak at 488nm. GFPmut1 fluoresces 35-fold more intensely than wtGFP when excited at 488nm, due to an increase in its extinction coefficient (Em) . This antibody is a rabbit polyclonal antibody raised against full-length eGFP and reactive against all variants of Aequorea victoria GFP such as S65T-GFP, RS-GFP, YFP, CFP and eGFP. Note: Do not add Azium (Sodium Azide or Smite) into the dilution buffer. Azium is the HRP inhibitor which decreases the enzyme activity of HRP.

Synonyms

CFP|eGFP|eYFP|GFP|GFP tag|YFP

UniProt

P42212

Host

Mouse

Reactivity

Paequorea victoria, recombinant protein

Immunogen

GFP tag

Target

GFP tag

Clonality

Monoclonal

Isotype

IgG1

Conjugation

HRP

Purification

Protein A+G purification

Dilution

WB: 1:2000-1:10000

Purity

≥95% as determined by SDS-PAGE

Form

Liquid

Molecular Weight

30 kDa

Storage Conditions

PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20°C for 12 months (Avoid repeated freeze / thaw cycles.)

Clone ID

0D8

Tested Applications

WB

Immunogen Sequence

1-238aa

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