HRP-GFP tag Monoclonal antibody
Product Specifications
Background
Green fluorescence protein (GFP) is a protein composed of 238 amino acid residues (26.9kDa) derived from the Jellyfish Aequorea victoria, which emits green light (emission peak at 509nm) when excited by blue light (excitation peak at 395nm) . GFP has become an invaluable tool in cell biology research, since its intrinsic fluorescence can be visualized in living cells.In contrast to wtGFP, EGFP has a single, strong, red-shifted excitation peak at 488nm. GFPmut1 fluoresces 35-fold more intensely than wtGFP when excited at 488nm, due to an increase in its extinction coefficient (Em) . This antibody is a rabbit polyclonal antibody raised against full-length eGFP and reactive against all variants of Aequorea victoria GFP such as S65T-GFP, RS-GFP, YFP, CFP and eGFP. Note: Do not add Azium (Sodium Azide or Smite) into the dilution buffer. Azium is the HRP inhibitor which decreases the enzyme activity of HRP.
Synonyms
CFP|eGFP|eYFP|GFP|GFP tag|YFP
UniProt
P42212
Host
Mouse
Reactivity
Paequorea victoria, recombinant protein
Immunogen
GFP tag
Target
GFP tag
Clonality
Monoclonal
Isotype
IgG1
Conjugation
HRP
Purification
Protein A+G purification
Dilution
WB: 1:2000-1:10000
Purity
≥95% as determined by SDS-PAGE
Form
Liquid
Molecular Weight
30 kDa
Storage Conditions
PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20°C for 12 months (Avoid repeated freeze / thaw cycles.)
Clone ID
0D8
Tested Applications
WB
Immunogen Sequence
1-238aa
Curated Selection
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