12-Hydroxyeicosatetraenoic acid ELISA Kit

12-Hydroxyeicosatetraenoic acid (12-HETE) is an eicosanoid. It is a metabolite of arachidonic acid produced by the action of the enzyme arachidonate 12-lipoxygenase. 12-HETE is a highly selective ligand used to label mu opioid receptors.

Aviva Systems Biology 12-Hydroxyeicosatetraenoic acid ELISA Kit (OKEH02579) is based on a competitive enzyme immunoassay technique. The microtiter well-plate in this kit has been pre-coated with an anti-12-Hydroxyeicosatetraenoic acid antibody. Sample or standards are added to the wells along with a fixed quantity of biotinylated 12-Hydroxyeicosatetraenoic acid and incubated. The 12-Hydroxyeicosatetraenoic acid found in the sample or standards competes with the biotinylated 12-Hydroxyeicosatetraenoic acid for limited binding sites on the immobilized anti-12-Hydroxyeicosatetraenoic acid antibody. Excess unbound biotinylated 12-Hydroxyeicosatetraenoic acid and sample or standard 12-Hydroxyeicosatetraenoic acid is washed from the plate. Avidin-HRP conjugate is added, incubated and washed. An enzymatic reaction is then produced through the addition of TMB substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of biotinylated 12-Hydroxyeicosatetraenoic acid captured in the well and inversely proportional to the amount of 12-Hydroxyeicosatetraenoic acid which was contained in the sample or standard.

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol