Tetrahydrofolic Acid ELISA Kit

Intra-assay Precision (Precision within an assay) : 3 samples with low, middle and high level Tetrahydrofolic Acid (THFA) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays) : 3 samples with low, middle and high level Tetrahydrofolic Acid (THFA) were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%

Tetrahydrofolic acid is a folic acid derivative. It is produced from dihydrofolic acid by dihydrofolate reductase. It is converted into 5,10-methylenetetrahydrofolate by serine hydroxymethyltransferase. It is a coenzyme in many reactions, especially in the metabolism of amino acids and nucleic acids. It acts as a donor of a group with one carbon atom. It gets this carbon atom by sequestering formaldehyde produced in other processes.

This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Tetrahydrofolic Acid (THFA) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Tetrahydrofolic Acid (THFA) and unlabeled Tetrahydrofolic Acid (THFA) (Standards or samples) with the pre-coated antibody specific to Tetrahydrofolic Acid (THFA) . After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Tetrahydrofolic Acid (THFA) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Tetrahydrofolic Acid (THFA) in the sample.

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Matrix Recovery Range (%) Average (%) serum (n=5) 89-97 92 EDTA plasma (n=5) 91-99 96 heparin plasma (n=5) 91-99 96 Matrix Recovery Range (%) Average (%) Matrix Recovery Range (%) Average (%) serum (n=5) 89-97 92 serum (n=5) 89-97 92 EDTA plasma (n=5) 91-99 96 EDTA plasma (n=5) 91-99 96 heparin plasma (n=5) 91-99 96 heparin plasma (n=5) 91-99 96

Component Amount Anti-Tetrahydrofolic Acid Microplate 96 Wells (12 x 8 Well Strips) Tetrahydrofolic Acid Lyophilized Standard 2 100X Tetrahydrofolic Acid Biotin Complex 120 uL 100X Avidin/HRP Conjugate 120 uL Standard Diluent 1 x 20 mL Biotin Complex Diluent 1 x 12 mL Avidin/HRP Conjugate Diluent 1 x 12 mL 30X Wash Buffer 1 x 20 mL TMB Substrate 1 x 9 mL Stop Solution 1 x 6 mL Component Amount Component Amount Anti-Tetrahydrofolic Acid Microplate 96 Wells (12 x 8 Well Strips) Anti-Tetrahydrofolic Acid Microplate 96 Wells (12 x 8 Well Strips) Tetrahydrofolic Acid Lyophilized Standard 2 Tetrahydrofolic Acid Lyophilized Standard 2 100X Tetrahydrofolic Acid Biotin Complex 120 uL 100X Tetrahydrofolic Acid Biotin Complex 120 uL 100X Avidin/HRP Conjugate 120 uL 100X Avidin/HRP Conjugate 120 uL Standard Diluent 1 x 20 mL Standard Diluent 1 x 20 mL Biotin Complex Diluent 1 x 12 mL Biotin Complex Diluent 1 x 12 mL Avidin/HRP Conjugate Diluent 1 x 12 mL Avidin/HRP Conjugate Diluent 1 x 12 mL 30X Wash Buffer 1 x 20 mL 30X Wash Buffer 1 x 20 mL TMB Substrate 1 x 9 mL TMB Substrate 1 x 9 mL Stop Solution 1 x 6 mL Stop Solution 1 x 6 mL