EGFR domain III Recombinant Antibody [h-R3 (Nimotuzumab) ] (OAAV00406)

Product Specifications

Gene Name

Ectodysplasin-A receptor

Gene Aliases

Anhidrotic ectodysplasin receptor 1; dl; downless; ectodermal dysplasia receptor; Ectodysplasin-A receptor; ED1R; ED3; ED5; EDA3; EDA-A1R; tumor necrosis factor receptor superfamily member EDAR.

Gene ID

13608

Accession Number

NP_034230.1ÃƒÂ¯Ã‚Â»Ã‚Â¿

Reactivity

Chicken, Dog, Rat, Human, Mouse

Immunogen

EDAR12 was prepared by immunixzing female OVE1B mice (with the Edar gene deleted) subcutaneously with mouse EDAR-Fc and positive hybridoma clones were screened for binding mEDAR by ELISA.

Clonality

Recombinant Monoclonal

Clone

EDAR12

Type

Recombinant Monoclonal Antibody

Purification

Protein A affinity purified

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Concentration

1 mg/ml

Dilution

Receptor for EDA isoform TAA, but not for EDA isoform TA-2 (By similarity) . May mediate the activation of NF-kappa-B and JNK. May promote caspase-independent cell death.

Format

Liquid// PBS with 0.02% Proclin300

Reconstitution

Store at 4° C for up to three (3) months. For longer storage, aliquot and store at -20° C.

Fragment

IgG1, kappa

Specificity

EDAR12 recognises and binds to CRD1 of the extracellular domain of mouse EDAR. EDAR3 cross-reacts with EDAR derived from human, dog, rat and chicken when EDAR is fused to the glycosylphosphatidylinositol anchor of TRAILR3. EDAR is the receptor for the TNF family ligand EDA1, which is a type II transmembrane protein possessing a collagen-like domain and a C-terminal TNF-homology domain. EDAR is important for the correct development of skin appendages including hair, teeth and eccrine sweat glands. LoF mutations in the Eda gene is known to cause XLHED (X-linked hypohidrotic ectodermal dysplasia), and results in abnormal development.