CD8 beta Recombinant Antibody [YTS 156.7.7] (OAAV00344)

Product Specifications

Gene Name

Integrin beta 7

Gene Aliases

Integrin beta-7; integrin beta-P; Ly69; M290 IEL antigen.

Gene ID

16421

Swiss Prot

P13612

Accession Number

NP_038594.2ÃƒÂ¯Ã‚Â»Ã‚Â¿

Immunogen

FIB504 was prepared by immunizing rats with TK1 cells.

Target

This chimeric rabbit antibody was made using the variable domain sequences of the original Rat IgG2a format, for improved compatibility with existing reagents, assays and techniques.

Clonality

Recombinant Monoclonal

Clone

FIB504

Type

Recombinant Monoclonal Antibody

Purification

Purified

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Dilution

Integrin alpha-4/beta-7 (Peyer patches-specific homing receptor LPAM-1) is involved in adhesive interactions of leukocytes. It is a receptor for fibronectin and recognizes one or more domains within the alternatively spliced CS-1 region of fibronectin. Integrin alpha-4/beta-7 is also a receptor for MADCAM1 and VCAM1. It recognizes the sequence L-D-T in MADCAM1. Integrin alpha-E/beta-7 is a receptor for E-cadherin.

Format

PBS with 0.02% Proclin 300.

Reconstitution

Store at 4C for up to 3 months. For longer storage, aliquot and store at -20C.

Fragment

IgG, kappa

Specificity

FIB504 recognizes an epitope on intergin beta-7 subunit that overlaps completely with the epitope defined by Fib27 and Fib30, and also partially with the epitope defined by FIB21 and FIB22. In the mouse beta-7 is selectively found on the majority of mature lymphocytes, whereas a small subpopulation of thymocytes and bone marrow cells express beta-7. Beta-7 is also expressed in variable amounts in CD4 T memory cells. Integrin molecules mediate cell-cell and cell-extracellular matrix adhesion and are involved in lymphocyte homing, leukocyte recruitment to inflammatory sites, myogenesis, hemopoiesis, and melanoma metastasis.