Bax (active monomer) Recombinant Antibody [6A7] (OAAV00079)

Apoptosis regulator BAX; Baxdelta2G9; Baxdelta2G9omega; Baxdelta2omega; BCL2 associated X protein; BCL2-associated X protein omega; bcl2-L-4; BCL2L4; bcl-2-like protein 4.

KLH conjugated to peptides corresponding to amino acids 3-16 of murine Bax (CGSGEQLGSGGPTSS), amino acids 3-16 of human Bax (CGSGEQPRGGGPTSS), and amino acids 12-24 shared by Bax from both species (CGPTSSEQIMKTGA) .

This chimeric rabbit antibody was made using the variable domain sequences of the original murine IgG1 format, for improved compatibility with existing reagents, assays and techniques.

Plays a role in the mitochondrial apoptotic process. Under normal conditions, BAX is largely cytosolic via constant retrotranslocation from mitochondria to the cytosol mediated by BCL2L1/Bcl-xL, which avoids accumulation of toxic BAX levels at the mitochondrial outer membrane (MOM) (PubMed:21458670) . Under stress conditions, undergoes a conformation change that causes translocation to the mitochondrion membrane, leading to the release of cytochrome c that then triggers apoptosis. Promotes activation of CASP3, and thereby apoptosis.

In the presence of nonionic detergent, the antibody avidly binds the monomeric form of Bax, but not Bax complexed with either Bcl-XL or Bcl-2. [6A7 ] Bax antibody only recognizes conformationally changed active Bax.