SAP97 Antibody, Clone G28: HRP

Rabbit Anti-Rat SAP97 Recombinant Monoclonal IgG Kappa

Product Specifications

Background

SAP97 (Synapse-Associated Protein 97), a member of the MAGUK family, is a critical scaffolding protein involved in the trafficking and localization of glutamate receptors, which are essential for synaptic plasticity and cognitive function. Mutations in the DLG1 gene encoding SAP97 have been linked to schizophrenia, with studies showing that these mutations enhance glutamatergic synapse strength in the dentate gyrus and impair episodic memory processing. SAP97 also mediates a non-conventional secretory pathway for NMDA receptors via dendritic Golgi outposts, highlighting its unique role in receptor sorting and synaptic regulation. Furthermore, SAP97 regulates the trafficking of ADAM10, a key α-secretase involved in amyloid precursor protein processing, implicating it in Alzheimer’s disease mechanisms. Our Anti-Rat SAP97 recombinant monoclonal antibody has been demonstrated to detect the target in rodent brain lysates and in cell culture lines.

Product Name Alternative

SAP97 Antibody, DLGH1 Antibody, DLGH 1 Antibody, DLG1 Antibody, DLG 1 Antibody, Synapse-associated protein 97 Antibody, Disks large homolog 1 Antibody, SAP-97 Antibody, SAP 97 Antibody

UNSPSC

12352203

UN Code

Non-hazardous

Hazard Statement

Non-hazardous

Gene ID

25252

Swiss Prot

Q62696

Accession Number

NP_036920.2

Host

Rabbit

Immunogen

Fusion protein, AA #1-104 (N-terminus) of rat SAP97.

Clone

G28

Conjugation

HRP

Tissue Specificity

Widely expressed. Brain, Heart, Muscle, Lung, Liver, Epithelial Cells

Type

Recombinant Monoclonal

Applications

WB | ICC-IF

Field of Research

Neuroscience | Cell Structure | Post-Synaptic Markers | Cell Signaling | Organelle Markers

Purification

MabSelect™ PrismA

Limit Of Detection

1 µg/ml was sufficient for detection of SAP97 on Rat Brain Membrane lysate.

Concentration

1mg/mL

Dilution

WB (1:1000), ICC/IF (1:100) ; optimal dilutions for assays should be determined by the user.; optimal dilutions for assays should be determined by the user.

Buffer

73.64mM Carbonate, 54.55mM Ethanolamine, 45.45mM Cyanoborohydride, 18.18mM Sodium Hydroxide, 0.23mM Citrate

Precautions

Not for use in humans. Not for use in diagnostics or therapeutics. For in vitro research use only.

Shipping Conditions

Blue Ice or 4ºC

Storage Conditions

Conjugated antibodies should be stored according to the product label

Specificity

Detects N-terminal region of SAP97. Does not cross-react against PSD95, Chapsyn-110, or SAP102

Background Reference 01

1. E. I., Marshall, J., Aoki, C., de Silva, T., Montgomery, J. M., Garner, C. C., & Green, W. N. (2009) . SAP97 and CASK mediate sorting of NMDA receptors through a previously unknown secretory pathway. Nature Neuroscience, 12, 1011–1019. https://doi.org/10.1038/nn.2362 2. Kay, Y., Tsan, L., Davis, E. A., Tian, C., Décarie-Spain, L., Sadybekov, A., Pushkin, A. N., Katritch, V., Kanoski, S. E., & Herring, B. E. (2022) . Schizophrenia-associated SAP97 mutations increase glutamatergic synapse strength in the dentate gyrus and impair contextual episodic memory in rats. Nature Communications, 13, 798. https://doi.org/10.1038/s41467-022-28430-5 3. Saraceno, C., Marcello, E., Di Marino, D., Borroni, B., Claeysen, S., Perroy, J., Padovani, A., Tramontano, A., Gardoni, F., & Di Luca, M. (2014) . SAP97-mediated ADAM10 trafficking from Golgi outposts depends on PKC phosphorylation. Cell Death and Disease, 5, e1542. https://doi.org/10.1038/cddis.2014.492