CASK Antibody, Clone PT55: RPE

CASK (calcium/calmodulin-dependent serine protein kinase) is a ~112 kDa member of the membrane-associated guanylate kinase (MAGUK) family, functioning as a critical scaffolding protein in neuronal development and synaptic organization. Structurally, CASK integrates multiple signaling domains—including a CaMK-like kinase domain, SH3 domain, PDZ domain, and a guanylate kinase (GUK) homology domain—enabling it to anchor transmembrane proteins to the cytoskeleton and intracellular signaling complexes. In the nervous system, CASK plays a pivotal role in stabilizing synaptic architecture by binding to neurexins at the presynaptic membrane, facilitating the assembly of pre- and postsynaptic structures. Beyond its cytoplasmic functions, a nuclear fraction of CASK translocates to the nucleus, where it acts as a transcriptional co-activator, influencing gene expression programs essential for neurodevelopment. Loss-of-function studies underscore CASK’s essential role: mice with CASK gene disruptions exhibit perinatal lethality, highlighting its necessity for survival. Additionally, CASK is expressed in non-neuronal tissues, including basal keratinocytes and developing hair follicles, suggesting broader roles in tissue development and wound repair. Emerging evidence links CASK dysfunction to neurodevelopmental and neurodegenerative disorders, including intellectual disability, microcephaly, and cerebellar atrophy. As a multifunctional regulator of synaptic integrity and gene expression, CASK is increasingly recognized as a key molecular target in neuroscience and neurodegeneration research.

CASK, Peripheral plasma membrane protein CASK, Calcium/calmodulin-dependent serine protein kinase, Calcium calmodulin-dependent serine protein kinase, LIN2, Lin-2 homolog, CAMGUK, CMG2, FGS4, MICPCH, MRXSNA, TNRC8, hCASK

WB (1:1000), IHC (1:100), ICC/IF (1:100) ; optimal dilutions for assays should be determined by the user.; optimal dilutions for assays should be determined by the user.

1. Hata, Y., Butz, S., & Südhof, T. C. (1996) . CASK: A novel dlg/PSD95 homolog with an N-terminal calmodulin-dependent protein kinase domain identified by interaction with neurexins. Journal of Neuroscience, 16 (8), 2488–2494. DOI: 10.1523/JNEUROSCI.16-08-02488.1996 2. Hsueh, Y. P., Wang, T. F., Yang, F. C., & Sheng, M. (2000) . Nuclear translocation and transcription regulation by the membrane-associated guanylate kinase CASK/LIN-2. Nature, 404 (6775), 298–302. DOI: 10.1038/35005118 3. Laverty, H. G., & Wilson, J. B. (1998) . Murine CASK is disrupted in a sex-linked cleft palate mouse mutant. Genomics, 53 (1), 29–41. DOI: 10.1006/geno.1998.5479 4. Ojeh, N., Pekovic, V., Jahoda, C., & Maatta, A. (2008) . The MAGUK-family protein CASK is targeted to nuclei of the basal epidermis and controls keratinocyte proliferation. Journal of Cell Science, 121 (16), 2705–2717. DOI: 10.1242/jcs.025643.