PSD-95 Antibody, Clone B14: RPE

Rabbit Anti-Human PSD-95 Recombinant Monoclonal IgG Kappa

Product Specifications

Background

PSD95 (Postsynaptic Density Protein 95), also known as SAP90, is a scaffolding protein in the membrane-associated guanylate kinase (MAGUK) family. It organizes the postsynaptic density by anchoring receptors, ion channels, and signaling molecules at excitatory synapses. PSD95 contains three PDZ domains, an SH3 domain, and a guanylate kinase-like domain. The PDZ domains mediate interactions with NMDA receptors, potassium channels, and neuroligins, facilitating synaptic signaling and plasticity. PSD95 also binds neuronal nitric oxide synthase, linking it to redox signaling. Through its role in AMPA receptor trafficking via Stargazin and TARPs, PSD95 influences synaptic strength and experience-dependent plasticity. Mutations in PSD95 are associated with autism spectrum disorders and cognitive deficits, underscoring its importance in learning and memory and its relevance in neurodegenerative disease research.

Product Name Alternative

PSD 95, PSD-95, PSD95, DLG4, SAP90, Synapse-associated protein 90, Postsynaptic density protein 95, Disks large homolog 4

UNSPSC

12352203

UN Code

Non-hazardous

Hazard Statement

Non-hazardous

Gene ID

1742

Swiss Prot

P78352

Accession Number

NP_001308004.1

Host

Rabbit

Immunogen

Fusion protein, AA #77-299 (PDZ domains 1 and 2) of human PSD95.

Clone

B14

Conjugation

RPE

Tissue Specificity

Brain

Type

Recombinant Monoclonal

Applications

WB | IHC | ICC/IF

Field of Research

Purification

AmMagTM Ultra AT Protein A MagBeads

Limit Of Detection

1 µg/ml was sufficient for detection of PSD95 on Rat Brain Membrane lysate.

Concentration

1mg/ml

Dilution

WB (1:1000), IHC (1:100), ICC/IF (1:100) ; optimal dilutions for assays should be determined by the user.; optimal dilutions for assays should be determined by the user.

Buffer

95.46mM Phosphate, 2.48mM MES and 2mM EDTA

Precautions

Not for use in humans. Not for use in diagnostics or therapeutics. For in vitro research use only.

Shipping Conditions

Blue Ice or 4ºC

Storage Conditions

Conjugated antibodies should be stored according to the product label

Specificity

Detects region of PSD95 containing PDZ domains 1 and 2. Does not cross-react with DLG1/SAP97, DLG2/Chapsyn-110 or DLG3/SAP102.

Background Reference 01

1. Chetkovich D.M., Bunn R.C., Kuo S.H., Kawasaki Y., Kohwi M., and Bredt D.S. (2002) J Neurosci. 22 (15) : 6415-25. 2. Cao J., Viholainen J.I., Dart C., Warwick H.K., Levland M.L. and Courtney M.J. (2005) J Cell Biol. 168 (1) : 117-26. 3. Kennedy M. (1997) Trends in Neurosci. 6: 264-268. 4. Irie M. et al. (1997) Science 277 (5331) : 1511-5. 5. Cai C. et al. (2006) J Biol Chem. 281: 4267-73. 6. Yao W.D. et al. (2004) Neuron 41: 625-38. 7. Cline H. (2005) Curr Biol. 15: R203-5.