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Recombinant GAPDH Monoclonal Antibody

Product Specifications

Background

GAPDH is an enzyme of 36kDa that catalyzes the sixth step of glycolysis and thus serves to break down glucose for energy and carbon molecules. Because the GAPDH gene is often stably and constitutively expressed at high levels in most tissues and cells, it is commonly used as a loading control for western blot and as a control for qPCR.

Abbreviation

GAPDH

UniProt

P16858

Host

Rabbit

Reactivity

Human; Mouse; Rat

Immunogen

Recombinant protein within Mouse GAPDH

Target

BARS-38; cb609; G3PDH; GAPD; KNC-NDS6; OCAS

Clonality

Recombinant

Clone

20B8

Conjugation

Unconjugated

Applications

WB; IHC; IF

Field of Research

Isotype; Loading Controls; Signal Transduction; Cancer; Metabolism

Purification

Protein A/G Purification

Concentration

1 mg/mL

Dilution

WB 1:20000-1:100000; IHC 1:2000-1:4000; IF 1:2000-1:4000

Buffer

TBS with 0.05% sodium azide, 0.05% protective protein and 40% glycerol, pH7.4

Shipping Conditions

The product is shipped with ice pack, upon receipt, store it immediately at the temperature recommended.

Storage Conditions

Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. Protected from prolonged exposure to light.

Calculated Molecular Weight

36kDa

Observed Molecular Weight

36kDa

Isotype

IgG

Available Sizes

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