Recombinant GAPDH Monoclonal Antibody
Product Specifications
Background
GAPDH is an enzyme of 36kDa that catalyzes the sixth step of glycolysis and thus serves to break down glucose for energy and carbon molecules. Because the GAPDH gene is often stably and constitutively expressed at high levels in most tissues and cells, it is commonly used as a loading control for western blot and as a control for qPCR.
Abbreviation
GAPDH
UniProt
P16858
Host
Rabbit
Reactivity
Human; Mouse; Rat
Immunogen
Recombinant protein within Mouse GAPDH
Target
BARS-38; cb609; G3PDH; GAPD; KNC-NDS6; OCAS
Clonality
Recombinant
Clone
20B8
Conjugation
Unconjugated
Applications
WB; IHC; IF
Field of Research
Isotype; Loading Controls; Signal Transduction; Cancer; Metabolism
Purification
Protein A/G Purification
Concentration
1 mg/mL
Dilution
WB 1:20000-1:100000; IHC 1:2000-1:4000; IF 1:2000-1:4000
Buffer
TBS with 0.05% sodium azide, 0.05% protective protein and 40% glycerol, pH7.4
Shipping Conditions
The product is shipped with ice pack, upon receipt, store it immediately at the temperature recommended.
Storage Conditions
Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. Protected from prolonged exposure to light.
Calculated Molecular Weight
36kDa
Observed Molecular Weight
36kDa
Isotype
IgG
Available Sizes
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