Rabies virus (RABV) Matrix protein (M) ELISA Kit

PRINCIPLE OF THE ASSAY This assay employs the quantitative sandwich enzyme immunoassay technique. An antibody specific for Rabies virus (RABV) Matrix protein (M) has been pre-coated onto a microplate. Standards or samples are pipetted into the wells and any Rabies virus (RABV) Matrix protein (M) present is bound by the immobilized antibody. After washing away any unbound substances, a biotin-labeled antibody specific for Rabies virus (RABV) Matrix protein (M) is added to the wells. After washing away any unbound substances, Streptavidin-HRP is added to the wells. Following a wash to remove any unbound enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of Rabies virus (RABV) Matrix protein (M) bound in the initial step. The color development is stopped and the intensity of the color is measured.