Anti-dsDNA Mouse IgG ELISA Kit

PRINCIPLE OF THE ASSAY This assay employs the quantitative indirect enzyme immunoassay technique. Ds DNA has been pre-coated onto a microplate. Standards or samples are pipetted into the wells and any Anti-dsDNA Mouse IgG present is bound by the immobilized protein. After washing away any unbound substances, a HRP-labeled Goat Anti-Mouse IgG antibody is added to the wells. Following a wash to remove any unbound enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of Anti-dsDNA Mouse IgG bound in the initial step. The color development is stopped and the intensity of the color is measured.