Human ST2 ELISA Kit

PRINCIPLE OF THE ASSAY This assay employs the quantitative sandwich enzyme immunoassay technique. An antibody specific for Human ST2 has been pre-coated onto a microplate. Standards or samples are pipetted into the wells and any Human ST2 present is bound by the immobilized antibody. After washing away any unbound substances, a biotin-labeled antibody specific for Human ST2 is added to the wells. After washing away any unbound substances, Streptavidin-HRP is added to the wells. Following a wash to remove any unbound enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of Human ST2 bound in the initial step. The color development is stopped and the intensity of the color is measured.