Secukinumab ELISA Kit

PRINCIPLE OF THE ASSAY This assay employs the quantitative indirect enzyme immunoassay technique. Recombinant Human IL17A has been pre-coated onto a microplate. Standards or samples are pipetted into the wells and any Secukinumab present is bound by the immobilized protein. After washing away any unbound substances, abiotin-labeled Mouse Anti-Human IgG antibody is added to the wells. After washing away any unbound substances, Streptavidin-HRP is added to the wells. Following a wash to remove any unbound enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of Secukinumab bound in the initial step. The color development is stopped and the intensity of the color is measured.