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Recombinant Protein L

Protein L is a multi-domain protein found on the surface of Peptostreptococcus magnus, also known as Peptostreptococcus magnus Protein L (PPL) . Unlike Protein A or Protein G, Protein L primarily binds to the kappa light chain of antibodies without interfering with the antigen-binding domain. This unique binding mechanism enables Protein L to interact with a broader range of antibody classes and subtypes, including human IgG, IgM, IgA, and IgD containing kappa light chains, as well as mouse IgG, IgM, rat IgG, Fab fragments, and single-chain variable fragments (scFv), among others.

Product Specifications

Product Name Alternative

Protein L, Peptostreptococcus magnus Protein L, PPL

Expression System

E. coli

Nature

Recombinant

Applications

Detection and purification of antibodies and antibody fragments (Fab, ScFv) that contain kappa light chains but not the Fc region.

Stability

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for one week. Store at -20 to -80°C for twelve months from the date of receipt.

Endotoxin

Please contact with the lab for this information.

Purity

>90% as determined by SDS-PAGE.

Form

Lyophilized

Buffer

Lyophilized from a solution in PBS pH 7.4, 1mM EDTA, 4% Trehalose, 1% Mannitol.

Reconstitution

Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details.

Shipping Conditions

In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.

Notes

For research use only.

Species

Peptostreptococcus magnus

Frequently Asked Questions

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