Recombinant Mouse TNFa/TNF-alpha Protein, No tag (Active)
Product Specifications
Product Name Alternative
Tumor necrosis factor, Cachectin, TNF-alpha, Tumor necrosis factor ligand superfamily member 2, TNF-a, Tumor necrosis factor, membrane form, N-terminal fragment, NTF, Intracellular domain 1, ICD1, Intracellular domain 2, ICD2, C-domain 1, C-domain 2, Tumor necrosis factor, soluble form, TNF, TNFA, TNFSF2
Accession Number
P06804
Expression System
E. coli
Nature
Recombinant
Applications
Bioactivity, ELISA, Immunogen, SDS-PAGE, WB
Stability
Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for one week. Store at -20 to -80°C for twelve months from the date of receipt.
Endotoxin
<1 EU/μg of the protein by the LAL method.
Purity
>90% as determined by SDS-PAGE.
Bioactivity
Recombinant Mouse TNFa/TNF-alpha (Cat #: AMB94401) induces cytotoxicity in the mouse fibroblast cells (L-929) in the presence of the metabolic inhibitor actinomycin D. The ED50 for this effect is typically 29-116 pg/mL, corresponding to a specific activity of 8.62x10^6-3.45x10^7 units/mg.
Form
Lyophilized
Buffer
Lyophilized from a solution in PBS pH 7.4, 5% Trehalose, 5% Mannitol.
Reconstitution
Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details.
Shipping Conditions
In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Notes
For research use only.
Species
Mus musculus (Mouse)
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