Recombinant Human SEMA5B Protein, C-hFc-His (Active)

Product Specifications

Product Name Alternative

SEMAG, Semaphorin-5B, SEMA5B, KIAA1445

Accession Number

Q9P283

Expression System

Mammalian Cells

Nature

Recombinant

Applications

Bioactivity, ELISA, Immunogen, SDS-PAGE, WB

Stability

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for one week. Store at -20 to -80°C for twelve months from the date of receipt.

Endotoxin

<0.1 EU/μg of the protein by the LAL method.

Purity

>90% as determined by SDS-PAGE.

Bioactivity

Measured by the ability of the immobilized protein to support the adhesion of human pancreatic cancer cells. When 5 x 10^4 cells/well are added to recombinant human SEMA5B (Cat #: AHJ71601) coated plates, cell adhesion is enhanced in a dose dependent manner after 1 hour at 37 °C. The ED50 for this effect is 0.7‑2.8 μg/mL.