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Recombinant Human SEMA5B Protein, C-hFc-His (Active)

Product Specifications

Product Name Alternative

SEMAG, Semaphorin-5B, SEMA5B, KIAA1445

Accession Number

Q9P283

Expression System

Mammalian Cells

Nature

Recombinant

Applications

Bioactivity, ELISA, Immunogen, SDS-PAGE, WB

Stability

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for one week. Store at -20 to -80°C for twelve months from the date of receipt.

Endotoxin

<0.1 EU/μg of the protein by the LAL method.

Purity

>90% as determined by SDS-PAGE.

Bioactivity

Measured by the ability of the immobilized protein to support the adhesion of human pancreatic cancer cells. When 5 x 10^4 cells/well are added to recombinant human SEMA5B (Cat #: AHJ71601) coated plates, cell adhesion is enhanced in a dose dependent manner after 1 hour at 37 °C. The ED50 for this effect is 0.7‑2.8 μg/mL.

Form

Lyophilized

Buffer

Lyophilized from a solution in PBS pH 7.4, 5% Trehalose, 5% Mannitol.

Reconstitution

Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details.

Shipping Conditions

In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.

Notes

For research use only.

Species

Homo sapiens (Human)

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