Recombinant Human HHIP Protein, C-His (Active)
Product Specifications
Product Name Alternative
HIP, HHIP, Hedgehog-interacting protein
Accession Number
Q96QV1
Expression System
Mammalian Cells
Nature
Recombinant
Applications
Bioactivity, ELISA, Immunogen, SDS-PAGE, WB
Stability
Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for one week. Store at -20 to -80°C for twelve months from the date of receipt.
Endotoxin
<0.1 EU/μg of the protein by the LAL method.
Purity
>90% as determined by SDS-PAGE.
Bioactivity
Measured by its ability to inhibit Sonic Hedgehog (Shh) induction of alkaline phosphatase production in mouse embryonic fibroblast cells (C3H10T1/2) . Recombinant human HHIP (Cat #: AHN57501) inhibits a constant dose of 20 ng/mL of recombinant human Sonic Hedgehog/Shh Protein, high activity in a mouse embryonic fibroblast cell (C3H10T1/2) alkaline phosphatase induction assay with an ED50 = 0.1-0.6 µg/mL.
Form
Lyophilized
Buffer
Lyophilized from a solution in PBS pH 7.4, 5% Trehalose, 5% Mannitol.
Reconstitution
Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details.
Shipping Conditions
In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Notes
For research use only.
Species
Homo sapiens (Human)
Curated Selection
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