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Recombinant Mouse CD62L/SELL Protein, C-hFc-His (Active)

Product Specifications

Product Name Alternative

L-selectin, Sell

Accession Number

Q3UV83

Expression System

Mammalian Cells

Nature

Recombinant

Applications

Bioactivity, ELISA, Immunogen, SDS-PAGE, WB

Stability

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for one week. Store at -20 to -80°C for twelve months from the date of receipt.

Endotoxin

<0.1 EU/μg of the protein by the LAL method.

Purity

>90% as determined by SDS-PAGE.

Bioactivity

Measured by the ability of the immobilized protein to support the adhesion of human colorectal adenocarcinoma cells (LS180) . When 5 x 104 cells/well are added to recombinant mouse CD62L/SELL (Cat #: AMD03301) coated plates, adhesion is induced in a dose dependent manner after a 1 hour incubation at 37 °C. The ED50 for this effect is 0.3‑1.2 μg/mL.

Form

Lyophilized

Buffer

Lyophilized from a solution in PBS pH 7.4, 5% Trehalose, 5% Mannitol.

Reconstitution

Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details.

Shipping Conditions

In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.

Notes

For research use only.

Species

Mus musculus (Mouse)

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