Recombinant Human CD31/PECAM1 Protein, C-His (Active)
Product Specifications
Product Name Alternative
PECAM-1, Platelet endothelial cell adhesion molecule, GPIIA', PECAM1, PECA1, EndoCAM, CD31
Accession Number
P16284
Expression System
Mammalian Cells
Nature
Recombinant
Applications
Bioactivity, ELISA, Immunogen, SDS-PAGE, WB
Stability
Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for one week. Store at -20 to -80°C for twelve months from the date of receipt.
Endotoxin
<0.1 EU/μg of the protein by the LAL method.
Purity
>90% as determined by SDS-PAGE.
Bioactivity
Measured by the ability of the immobilized protein to support the adhesion of human acute T cell leukemia cells (Jurkat) . When 8x10^4 cells/well are added to CD31/PECAM1 (Cat #: AHD16401) coated plates (5μg/mL, 100 μL/well) in the presence of 10 μg/mL PHA, approximately 60-70% will adhere after 30 minutes at 37°C.
Form
Lyophilized
Buffer
Lyophilized from a solution in PBS pH 7.4, 5% Trehalose, 5% Mannitol.
Reconstitution
Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details.
Shipping Conditions
In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Notes
For research use only.
Species
Homo sapiens (Human)
Curated Selection
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