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Recombinant Human GZMA/Granzyme A Protein, C-10*His (Active)

Product Specifications

Product Name Alternative

H factor, CTLA3, Cytotoxic T-lymphocyte proteinase 1, CTL tryptase, Fragmentin-1, HFSP, HF, Granzyme A, Granzyme-1, GZMA, Hanukkah factor

Accession Number

P12544

Expression System

Mammalian Cells

Nature

Recombinant

Applications

Bioactivity, ELISA, Immunogen, SDS-PAGE, WB

Stability

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for one week. Store at -20 to -80°C for twelve months from the date of receipt.

Endotoxin

<0.1 EU/μg of the protein by the LAL method.

Purity

>90% as determined by SDS-PAGE.

Bioactivity

Measured by its ability to cleave a colorimetric peptide substrate, N-carbobenzyloxy-Gly-ArgThioBenzyl ester (Z-GR-SBzl), in the presence of 5,5’Dithio-bis (2-nitrobenzoic acid) (DTNB) . The specific activity is >5,000 pmol/min/µg, as measured under the described conditions.

Form

Lyophilized

Buffer

Lyophilized from a solution in PBS pH 7.4, 5% Trehalose, 5% Mannitol.

Reconstitution

Reconstitute in sterile water for a stock solution.

Shipping Conditions

In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.

Notes

For research use only.

Species

Homo sapiens (Human)

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