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Recombinant Human OPN/Spp1 Protein, C-His (Active)

Product Specifications

Product Name Alternative

Osteopontin, BNSP, Uropontin, OPN, SPP-1, Secreted phosphoprotein 1, Bone sialoprotein 1, Nephropontin, Urinary stone protein, SPP1

Accession Number

P10451

Expression System

Mammalian Cells

Nature

Recombinant

Applications

Bioactivity, ELISA, Immunogen, SDS-PAGE, WB

Stability

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for one week. Store at -20 to -80°C for twelve months from the date of receipt.

Endotoxin

<0.1 EU/μg of the protein by the LAL method.

Purity

>90% as determined by SDS-PAGE.

Bioactivity

Measured by the ability of the immobilized protein to support the adhesion of human embryonic kidney cells (HEK293) . Recombinant human OPN/Spp1 (Cat #: AHC81101) coated plate, cell adhesion is enhanced in a dose-dependent manner after 1 hour incubation at 37 °C. The ED50 for this effect is 0.2-0.8 µg/mL.

Form

Lyophilized

Buffer

Lyophilized from a solution in PBS pH 7.4, 5% Trehalose, 5% Mannitol.

Reconstitution

Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details.

Shipping Conditions

In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.

Notes

For research use only.

Species

Homo sapiens (Human)

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