Recombinant Human OPN/Spp1 Protein, C-His (Active)

Product Specifications

Product Name Alternative

Osteopontin, BNSP, Uropontin, OPN, SPP-1, Secreted phosphoprotein 1, Bone sialoprotein 1, Nephropontin, Urinary stone protein, SPP1

Accession Number

P10451

Expression System

Mammalian Cells

Nature

Recombinant

Applications

Bioactivity, ELISA, Immunogen, SDS-PAGE, WB

Stability

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for one week. Store at -20 to -80°C for twelve months from the date of receipt.

Endotoxin

<0.1 EU/μg of the protein by the LAL method.

Purity

>90% as determined by SDS-PAGE.

Bioactivity

Measured by the ability of the immobilized protein to support the adhesion of human embryonic kidney cells (HEK293) . Recombinant human OPN/Spp1 (Cat #: AHC81101) coated plate, cell adhesion is enhanced in a dose-dependent manner after 1 hour incubation at 37 °C. The ED50 for this effect is 0.2-0.8 µg/mL.