Recombinant Human NTN1/Netrin-1 Protein, C-His (Active)
Product Specifications
Product Name Alternative
Netrin-1, NTN1, Epididymis tissue protein Li 131P, NTN1L
Accession Number
O95631
Expression System
Mammalian Cells
Nature
Recombinant
Applications
Bioactivity, ELISA, Immunogen, SDS-PAGE, WB
Stability
Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for one week. Store at -20 to -80°C for twelve months from the date of receipt.
Endotoxin
<0.1 EU/μg of the protein by the LAL method.
Purity
>90% as determined by SDS-PAGE.
Bioactivity
Measured by its binding ability in a functional ELISA. When recombinant Rat UNC5H2 is immobilized at 5 μg/mL, recombinant human NTN1/Netrin-1 (Cat #: AHB70501) binds with an apparent Kd <1 nM. Measured in a cell proliferation assay using rat schwannoma cells (RT4‑D6P2T) . The ED50 for this effect is 0.5-2.0 μg/mL.
Form
Lyophilized
Buffer
Lyophilized from a solution in PBS pH 7.4, 5% Trehalose, 5% Mannitol.
Reconstitution
Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details.
Shipping Conditions
In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Notes
For research use only.
Species
Homo sapiens (Human)
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